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KMID : 0903520070500040308
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 4 p.308 ~ p.315
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Distribution and Extraction Condition of Endoprotease and Exopeptidase from Viscera of Illex argentinus
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Kim Hye-Suk
Heu Min-Soo
Kim Jin-Soo
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Abstract
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For the effective use of squid processing by-products as food resources, the distribution and the extraction condition of endoprotease and exopeptidase from viscera of Illex argentinus were investi- gated. Crude protein and lipid contents of viscera of Illex argentinus were 17.2% and 16.9%, respec- tively. Regardless of kinds of extraction solution (water, 1% NaCl, 1% KCl and 1% NaCl- KCl) and extraction times (1-20 h), endoprotease activities from viscera of Illex argentinus on Hb, casein and azocasein (pH 6.0) were higher than those on casein and azocasein of the other pHs, thus indicating that the distribution of protein hydrolysing protease is distinctive in the weak acid pH range. Exopep- tidase activities against LeuPNA and ArgPNA at pH 7.5 were relatively higher than endoprotease activity of the same pH. The results suggested that exopeptidase among proteases from viscera of Illex argentinus was reasonable for application in food industry compared to endoprotease. The activity in enzymes from viscera of Illex argentinus was the highest in the exopeptidase extracted with deionized distilled water at room temperature for 6-8 h. The optimal reaction conditions of crude enzyme from viscera of Illex argentinus were 7.5 for pH and 50-55o C for temperature.
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KEYWORD
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squid, squid viscera, endoprotease, exopeptidase, proteases, Illex argentinus
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